AB5 ADP-ribosylating toxins: comparative anatomy and physiology

Arginine-Specific Mono ADP-Ribosylation In Vitro of Antimicrobial Peptides by ADP-Ribosylating Toxins

Among the several toxins used by pathogenic bacteria to target eukaryotic host cells, proteins that exert ADP-ribosylation activity represent a large and studied family of dangerous and potentially lethal toxins. These proteins alter cell physiology catalyzing the transfer of the ADP-ribose unit from NAD to cellular proteins involved in key metabolic pathways. In the present study, we tested th...

Docking and virtual screening of novel inhibitors for mono-ADP-ribosylating toxins

ADP-ribosyltransferases (ADP-RTs) are a family of enzymes secreted by pathogenic bacteria. They catalyse the hydrolysis of NAD+ and the transfer of the ADPribosyl group onto specific target proteins [1,2]. Figure 1 Although ADP-RTs are important drug targets, only few inhibitors are known so far. The high selectivity of these inhibitors suggest different mechanism of binding to the ADP-RTs acti...

Membrane translocation of binary actin-ADP-ribosylating toxins from Clostridium difficile

26 Some hypervirulent strains of Clostridium difficile produce the binary actin-ADP-ribosylating 27 toxin CDT in addition to the Rho-glucosylating toxins A and B. It has been suggested that the 28 presence of CDT increases the severity of the C. difficile-associated diseases including 29 pseudomembranous colitis. CDT contains a binding and translocation component CDTb, which 30 mediates the tra...

The ADP-Ribosylating CTA1-DD Adjuvant

Influence of DPH1 and DPH5 Protein Variants on the Synthesis of Diphthamide, the Target of ADP-Ribosylating Toxins

The diphthamide on eukaryotic translation elongation factor 2 (eEF2) is the target of ADPribosylating toxins and -derivatives that serve as payloads in targeted tumor therapy. Diphthamide is generated by seven DPH proteins; cells deficient in these (DPHko) lack diphthamide and are toxin-resistant. We have established assays to address the functionality of DPH1 (OVCA1) and DPH5 variants listed i...